Reproduction Abstracts

Introduction: Spermatogenesis is a complex process in which spermatogonial stem cells (SSCs) self-renew, develop to differentiated spermatogonia, and then give rise to meiotic spermatocytes. Functional sperm are ultimately differentiated from postmeiotic haploid spermatids. Cell culture methods representing this process will facilitate analyzing molecular function and imaging molecules. Here we describe consecutive two culture methods by which zebrafish SSCs enable to differen...

Introduction: Testicular tumors not only facilitate analyses of tumorigenesis in testes but also are a good resource for culturing spermatogonial stem cells (SSCs) that allow us to manipulate genetic modification. In zebrafish, a hypertrophied tumorous testis happens to be found occasionally, but no method for maintenance of the individual tumorous testis has prevented systematic analyses of the characters and the practical use. We describe here the method for maintenance of t...